Chromosomal Microarray Analysis for Research
The constitutional chromosomal microarray analysis and the neoplasia chromosomal microarray analysis can both be ordered as part of research studies. Call the Laboratory Director (206-685-1170) for more details.
Turnaround Time: 28 days from receipt of adequate sample
Test Requisition: Research Service Request Form
Constitutional CMA Specimen Types:
- Peripheral Blood: 3-4 ml for adults or 1-2 mL for infants in EDTA (lavender top vacutainer). Keep at room temperature.
- Cord Blood: 1-2 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Products of Conception and Fetal Tissue: For products of conception, send villi when identifiable. If material is small and unidentifiable, send entire sample. For fetal tissue, send fascia lata, lung, or kidney if possible. Fresh tissue: 30-50mg in sterile transport media containing antibiotics (e.g. Alpha-MEM media, RPMI) or two T-25 flasks 80-90% confluent. Keep refrigerated. Frozen tissue (-20 degrees): 30-50 mg. Remove media before freezing. Keep frozen (-20 degrees) during transport. FFPE tissue: Please send curls or slides if possible. Contact lab if block is the only option. If sample is mixed maternal and fetal, send 10 unstained 10μm FFPE slides and one image of an H&E stained slide that has the area of villi marked. If sample is entirely fetal, send 10 x 10μm curls in two 1.5 mL tubes (5 per tube). Send at 20-25°C and protect from excessive heat.
- Amniotic Fluid: 15-20ml of amniotic fluid (Discard the first 1mL of fluid to minimize risk of maternal cell contamination) OR two T-25 flasks that are 80-90% confluent. Keep at room temperature.
- Chorionic Villi: Two T-25 flasks that are 80-90% confluent. Contact lab to discuss direct testing of cleaned but uncultured villi. Keep at room temperature.
- DNA: 3-10 micrograms (minimum 1 microgram) at a concentration of 100 nanograms per microliter (or higher), suspended in water. Keep at room temperature.
- Saliva: 0.5mL in Oragene•ONE(ON-500) Collection Kit. Keep at room temperature.
Neoplasia Specimen Types:
- Leukemic Blood: 1-3 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Bone Marrow: 1-3 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Fresh Frozen Tumor Tissue: 50-150mg (0.15-2.0 cm3) fresh tissue snap-frozen. Store at -20°C. Ship on minimum of 10 lb of dry ice in insulated container by overnight courier.
- FFPE Tumor Slides: If tumor content is <70% send 10 unstained 10μm FFPE slides and one image of an H&E stained slide that has the area of tumor marked. If tumor content is >70% send 10 x 10μm curls in two 1.5 mL tubes (5 per tube). Include a copy of corresponding surgical pathology report. Mail slides in a protected mailing container at room temperature and protect from excessive heat.
- FFPE Tumor Block: Please send curls or slides if possible. Contact lab if block is the only option.
- Solid Tumor Cultures: Two T-25 flasks that are 80-100% confluent. Keep at room temperature.
- Bone Core: Contact the lab (206-598-4488).
- Lymph Node: Contact the lab (206-598-4488).
- DNA: 3-10 micrograms (minimum 1 microgram) at a concentration of 100 nanograms per microliter (or higher), suspended in water. Keep at room temperature.
Deliver to laboratory as soon as possible. Specimens are accepted Monday through Friday.
Ship sample overnight (Monday to Thursday) to:
Clinical Genomics Laboratory
University of Washington Health Sciences Building
Room H-561
1959 NE Pacific St
Seattle, WA 98195
Phone 206-616-4062