Flow Cytometry – Paraffin Specimens


DNA Content/Cell Cycle Analysis

Test Guide

The Clinical Flow Cytometry laboratory performs DNA content and cell cycle analyses of solid tissue specimens. The laboratory’s special expertise is in DNA content measurements of premalignant diseases of the GI tract, such as Barrett’s esophagus and ulcerative colitis, where the finding of DNA content abnormalities is associated with elevated cancer risk, and the determination of ploidy in the differential diagnosis of molar pregnancies.

Specimen Requirements

Paraffin block most representative of tumor to be analyzed and with highest ratio of tumor cells to normal. (Including a recent H&E reduces process time).

Blocks and/or slides are returned.

Special Instructions

Ship the same day on wet ice.
Ship overnight on dry ice.
A Phone call to notify laboratory of shipment is appreciated. Please do not ship for weekend or holiday delivery.

The label on each sample should include the patient name, identification number, and location of the biopsy site (in centimeters distal to the proximal).

Proven value:

    • Barrett’s Esophagus
    • Bladder
    • Breast Carcinoma
    • Endometrial Carcinoma
    • Molar pregnancy (products of conception)
    • Non-Hodgkin’s Lymphoma
    • Ovarian Carcinoma
    • Prostate
    • Ulcerative Colitis
Probable value:

  • Adrenal Cortical Neoplasms
  • Chondrosarcoma/Osteogenic Sarcoma
  • Colon Carcinoma
  • Glioma ?
  • Melanoma ?
  • Multiple Myeloma
  • Renal Cell Carcinoma
  • Soft tissue Sarcoma
  • Squamous Carcinomas of Lung
  • Squamous Cell Carcinoma of Cervix ?
Uncertain value:

  • Carcinoid Tumors
  • Meningioma
  • Mesothelioma
  • Thyroid Neoplasms


How to Order

Please complete the DNA Flow Cytometry Requisition form.  Include description of tissue source and copy of histologic diagnosis.

2.  Prepare the sample for submission (see Special Instructions above)

3.  Ship or carry the sample (see Special Instructions) to:

UW Medicine Pathology
Clinical Flow Cytometry Laboratory
1959 NE Pacific St,  Room BB220
Seattle,  WA  98195
phone  206-598-6400     fax  206-598-8049

     4.  Results returned 6 days from receipt.  Report contains results including histogram, interpretation and supporting literature references.

4.  Contact with questions:

              Tom Small  ph:  (206) 598-6415

CPT Code & Cost

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University of Washington, UW Medicine Pathology.