Y Chromosome Microdeletion Analysis

Genes

AMELY, CDY1, DAZ, DBY, HSFY1, RBMY1, SRY, USP9Y

Test Guide

Small deletions of the Y chromosome are found in 5-13% of men who are otherwise healthy but have no sperm, a low sperm count, or abnormal sperm shape or movement.

Methodology

This PCR-based analysis assesses 14 different regions along the length of the Y chromosome and will detect all of the microdeletions associated with male infertility, including the AZFa and AZFb/c regions. This analysis exceeds the minimum requirements outlined by the European Academy of Andrology (EAA) and the European Molecular Genetics Quality Network (EMQN).

Limitations of Methodology: Y chromosome microdeletion analysis via PCR will not detect point mutations or small intragenic mutations. Such mutations have been reported but are a very rare cause of male infertility.

Specimen Requirements

Specimen Types:

  • Peripheral Blood: 5 ml in EDTA (lavender top vacutainer).
  • DNA: 3-10 micrograms of DNA suspended in TE-buffer.
  • Saliva: 0.5mL in Oragene•ONE(ON-500) Collection Kit.

    Keep samples at room temperature.

Special Instructions

Return of Results:  Results will be faxed to the number(s) provided on the requisition form.

Turnaround Time: 7 to 14 days from receipt of adequate sample.

How to Order

Please follow these steps:

Test kits and return shipping are available, as needed.  Please call 206-598-8684 to request test kits.

1. Complete CCL Constitutional Test Request Form, including billing information.
2. Collect sample.
3. Deliver to Clinical Cytogenomics Laboratory as soon as possible.  Specimens are accepted 7 days a week.

Ship sample overnight to:

c/o Cytogenetics and Genomics Laboratory
University of Washington Medical Center
Room NW-125, Box 356100
1959 NE Pacific St
Seattle, WA 98195-6100

Note: Orders cancelled after they have been submitted will incur a prorated charge based on the work that has been completed as of the time of cancellation.

CPT Code & Cost

81403
$660

© 2017 UW Medicine Center For Precision Diagnostics. All rights reserved.
University of Washington, UW Medicine Pathology.
Schildbach Design, Web Designer & Logo Designer