Chromosomal Microarray Analysis for Research
The constitutional chromosomal microarray analysis and the neoplasia chromosomal microarray analysis can both be ordered as part of research studies. Call the Laboratory Director (206-685-1170) for more details.
Turnaround Time: 28 days from receipt of adequate sample
Test Requisition: Research Service Request Form
Constitutional CMA Specimen Types:
- Peripheral Blood: 3-5 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Cord Blood: 1-2 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Products of Conception and Fetal Tissue: 30-50mg fresh tissue in sterile transport media or two T-25 flasks 80-100% confluent. If identifiable tissue in products of conception, send chorionic villi. If material is small and unidentifiable, send entire sample. For stillbirths, send fascia lata, lung, or kidney if possible. Keep refrigerated.
- Amniotic Fluid: 15-20ml of amniotic fluid OR two T-25 flasks that are 80-100% confluent. Keep at room temperature.
- Chorionic Villi: Two T-25 flasks that are 80-100% confluent. Keep at room temperature.
- DNA: 3-10 micrograms of DNA (minimum 1 microgram) at a concentration of 100 nanograms per microliter (or higher) suspended in TE-buffer. Keep at room temperature.
- Saliva: 0.5mL in Oragene•ONE(ON-500) Collection Kit. Keep at room temperature.
Neoplasia Specimen Types:
- Leukemic Blood: 1-3 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Bone Marrow: 1-3 ml in EDTA (lavender top vacutainer). Keep at room temperature.
- Fresh Frozen Tumor Tissue: 50-150mg (0.15-2.0 cm3) fresh tissue snap-frozen. Store at -20°C. Ship on minimum of 10 lb of dry ice in insulated container by overnight courier.
- FFPE Tumor Slides: 10 unstained 5μm FFPE slides containing adequate amounts of tumor with areas of tumor marked. Include a copy of corresponding surgical pathology report. Mail slides in a protected mailing container at 20-25°C and protect from excessive heat.
- FFPE Tumor Block: Tissue block containing adequate amounts of tumor with areas of tumor marked. Include a copy of corresponding surgical pathology report. Mail at 20-25°C and protect from excessive heat.
- Solid Tumor Cultures: Two T-25 flasks that are 80-100% confluent. Keep at room temperature.
- Bone Core: Contact the lab (206-598-4488).
- Lymph Node: Contact the lab (206-598-4488).
- DNA: 3-10 micrograms of DNA suspended in TE-buffer.
Deliver to laboratory as soon as possible. Specimens are accepted 7 days a week.
Ship sample overnight (Monday to Thursday) to:
Clinical Genomics Laboratory
University of Washington Health Sciences Building
Room H-561
1959 NE Pacific St
Seattle, WA 98195
Phone 206-598-4488